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P0325LexusLexus RX HubAll Lexus P0325Moderate Severity

P0325 on Lexus RX: Fix & DLC Location

Factory diagnostic troubleshooting wire for fault code P0325 (P0325: Knock Sensor 1 Circuit Malfunction (Bank 1)) specifically on the Lexus RX (2020–2026, 2.4L Turbo (RX 350 275HP) / 2.5L Hybrid / 2.4L Turbo Performance Hybrid (RX 500h 366HP)).

Lexus RX
DIY Parts Estimate
$180 - $420 (DIY: $40 - $90)
RX OEM Fitment
Shop Labor Quote
$180 – $380
Based on $135/hr labor
Drivability Status
Drivable (Short Trip)
Moderate urgency level
Est. DIY Time
45 – 90 Min
Basic hand tools
Check P0325 On Another Lexus Model:
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1. Symptoms of P0325 on Lexus RX

When the Engine Control Module (ECM/PCM) on your Lexus RX (2.4L Turbo (RX 350 275HP) / 2.5L Hybrid / 2.4L Turbo Performance Hybrid (RX 500h 366HP)) logs fault code P0325, drivers commonly observe:

System alert or indicator light illuminated (P0325) on Lexus RX (2.4L Turbo (RX 350 275HP) / 2.5L Hybrid / 2.4L Turbo Performance Hybrid (RX 500h 366HP))
Intermittent performance throttling or unexpected operation halts on Lexus RX (2.4L Turbo (RX 350 275HP) / 2.5L Hybrid / 2.4L Turbo Performance Hybrid (RX 500h 366HP))
Audible sensor relay clicking or anomalous status telemetry in dashboard logs on Lexus RX (2.4L Turbo (RX 350 275HP) / 2.5L Hybrid / 2.4L Turbo Performance Hybrid (RX 500h 366HP))
Degraded operational throughput or error dialog popups on startup on Lexus RX (2.4L Turbo (RX 350 275HP) / 2.5L Hybrid / 2.4L Turbo Performance Hybrid (RX 500h 366HP))
Readiness monitors failing state emissions or system compliance audits on Lexus RX (2.4L Turbo (RX 350 275HP) / 2.5L Hybrid / 2.4L Turbo Performance Hybrid (RX 500h 366HP))
Lexus RX OBD-II Port Location
The 16-pin diagnostic connector is located Driver footwell above brake pedal assembly. Plug in your scan tool to verify freeze-frame sensor values.

2. Most Likely Causes on Lexus RX

Historical warranty claims and technical repair bulletins for Lexus RX point to the following failure distribution:

55%
Component aging, oxidation, or degraded internal circuit element (Piezoelectric Knock Sensor) (Lexus OEM Part)
25%
Wiring harness abrasion, loose terminal pin, or ground voltage float (Lexus OEM Part)
12%
Outdated firmware microcode or incompatible software driver update (Lexus OEM Part)
8%
Thermal stress or excessive operational duty-cycle fatigue (Lexus OEM Part)
8%
Contamination from carbon buildup, moisture ingress, or dirty filtration (Lexus OEM Part)

3. Step-by-Step DIY Repair Guide for Lexus RX

01
Connect Diagnostic Scanner / Logging Monitor to Retrieve Telemetry

Interface with the diagnostic port or system console using verified scanning hardware. Record freeze-frame data, active sub-codes, and timestamps to isolate whether P0325 is static or intermittent. on the Lexus RX equipped with 2.4L Turbo (RX 350 275HP) / 2.5L Hybrid / 2.4L Turbo Performance Hybrid (RX 500h 366HP).

Required Tool: 12mm deep socket, Torque wrench (20 Nm)
02
Perform Physical & Electrical Circuit Inspection

Visually inspect the physical component (Piezoelectric Knock Sensor), connector pins, and associated wiring harnesses for signs of corrosion, thermal melting, or loose grounding clips. on the Lexus RX equipped with 2.4L Turbo (RX 350 275HP) / 2.5L Hybrid / 2.4L Turbo Performance Hybrid (RX 500h 366HP).

Required Tool: Multimeter, Flashlight, Contact Cleaner Spray
03
Component Testing & Resistance / Voltage Verification

Measure reference voltages and resistance thresholds against manufacturer factory specifications. Replace any component failing nominal impedance tolerances. on the Lexus RX equipped with 2.4L Turbo (RX 350 275HP) / 2.5L Hybrid / 2.4L Turbo Performance Hybrid (RX 500h 366HP).

Required Tool: 12mm deep socket, Torque wrench (20 Nm)
04
Execute Component Replacement or Software Configuration Patch

Install genuine OEM replacement hardware or execute registry / service commands. Ensure all mounting bolts are torqued to factory specifications. on the Lexus RX equipped with 2.4L Turbo (RX 350 275HP) / 2.5L Hybrid / 2.4L Turbo Performance Hybrid (RX 500h 366HP).

Required Tool: Standard Hand Tool Set / Terminal Commands
05
Clear Diagnostic Trouble Code & Complete Verification Drive Cycle

Erase the fault code from non-volatile memory. Perform a standard verification cycle (e.g. 15-minute mixed drive or system stress benchmark) to ensure monitors reset to 'Ready' state without recurrent fault flags. on the Lexus RX equipped with 2.4L Turbo (RX 350 275HP) / 2.5L Hybrid / 2.4L Turbo Performance Hybrid (RX 500h 366HP).

Required Tool: OBD-II Code Reader / Diagnostic Suite

Other Common Fault Codes on Lexus RX

Frequently diagnosed powertrain, emissions, and sensor trouble codes logged on the Lexus RX:

4. Lexus RX P0325 FAQs

What causes code P0325 to trigger on a Lexus RX?

On the Lexus RX (2.4L Turbo (RX 350 275HP) / 2.5L Hybrid / 2.4L Turbo Performance Hybrid (RX 500h 366HP)), P0325 is most frequently caused by Component aging, oxidation, or degraded internal circuit element (Piezoelectric Knock Sensor) or Wiring harness abrasion, loose terminal pin, or ground voltage float.

Where is the OBD-II port located on my Lexus RX?

On the Lexus RX, the 16-pin DLC diagnostic connector is located Driver footwell above brake pedal assembly. Plug in any standard OBD-II scanner with the ignition in the ON position to read freeze-frame parameters.

Can I safely drive my Lexus RX with code P0325?

Yes, for temporary operations. However, resolve promptly to maintain optimal efficiency and prevent cascade failures. Given that P0325 carries a Moderate severity rating, we advise avoiding heavy towing or aggressive throttle inputs until sensor readings are verified.